Tang, X. Q., Bi, H., Feng, J. Q., and Cao, J. G. Effect of curcumin on multidrug resistance in resistant human gastric carcinoma cell line SGC7901/VCR. Phase IIa clinical trial of curcumin for the prevention of colorectal neoplasia. Mechanism(s) of turmeric-mediated protective effects against benzo(a)pyrene-derived DNA adducts. Nonsteroidal anti-inflammatory agents differ in their ability to suppress NF-kappaB activation, inhibition of expression of cyclooxygenase-2 and cyclin D1, and abrogation of tumor cell proliferation. Therefore, it may be safe to conclude that 1000mg of Curcumin and 5-10mg of Bioperine will not reach the threshold to inhibit testicular 17beta-hydroxysteroid dehydrogenase 3. Furtheremore, The potencies of inhibiting 17b-HSD3 in human testis microsomes for curcumin was 67.3 uM . Curcumin has also been found to inhibit testicular 17beta-hydroxysteroid dehydrogenase 3 potentially having a negative impact on testicular testosterone synthesis. Now I assume Men & Women metabolise Estrogen in the same manner, therefore one can extrapolate and assume a similar outcome for serum Estrogen levels in Men. However, grapefruit juice, a potent CYP3A4 inhibitor was actually shown clinically to reduce endogenous serum Estrogen levels in Postmenopausal Women . Estrogen is a substrate of CYP3A4 and logically I originally concluded that inhibiting CYP3A4 will impair Estrogen clearance from the body, potentially leading to downregulation of the HPTA and lowering of testosterone. I personally am using a formula with 5mg of Bioperine with 1000mg of Curcumin as I am currently taking prescription drugs and want to be on the safe side. Subconfluent LNCaP and 22Rv1 cells were treated with curcumin for 24 h. Total RNA of cells were extracted with RNA‐Bee isolation reagent (Tel‐Test, Oxfordshire) according to the manufacturer's instructions. To quantify the apoptosis induced by curcumin, we measured caspase‐3/7 activity consistent with apoptosis by ApoLive‐Glo Multiplex assay (Promega). In apoptotic cells, JC‐1 remains in the cytoplasm in a green fluorescent monomeric form reflecting a collapse of the mitochondrial membrane potential and an inability of JC‐1 to accumulate within the mitochondria. Apoptotic cells were directly visualized with inverted fluorescence microscopy (Olympus DP72, Tokyo, Japan). To verify the mechanism by which curcumin can induce apoptosis, we measured mitochondrial membrane potential changes in LNCaP cells. Gopalan, B., Goto, M., Kodama, A., and Hirose, T. Supercritical carbon dioxide extraction of turmeric (Curcuma longa). Park, E. J., Jeon, C. H., Ko, G., Kim, J., and Sohn, D. H. Protective effect of curcumin in rat liver injury induced by carbon tetrachloride. Choudhary, D., Chandra, D., and Kale, R. K. Modulation of radioresponse of glyoxalase system by curcumin. Chan, M. M., Ho, C. T., and Huang, H. I. Effects of three dietary phytochemicals from tea, rosemary and turmeric on inflammation-induced nitrite production. Appiah-Opong, R., Commandeur, J. N., Vugt-Lussenburg, B., and Vermeulen, N. P. Inhibition of human recombinant cytochrome P450s by curcumin and curcumin decomposition products. Choi, B. H., Kim, C. G., Lim, Y., Shin, S. Y., and Lee, Y. H. Curcumin down-regulates the multidrug-resistance mdr1b gene by inhibiting the PI3K/Akt/NF kappa B pathway. Junyaprasert, V. B., Soonthornchareonnon, N., Thongpraditchote, S., Murakami, T., and Takano, M. Inhibitory effect of Thai plant extracts on P-glycoprotein mediated efflux. In addition, many of curcumin's biological effects involve key components of signal transduction pathways within cancer cells, including prostate cancer cells. Taken together, these results suggest that curcumin's natural bioactive compounds could have potent anticancer properties due to suppression of androgen production, and this could have therapeutic effects on prostate cancer. Curcumin decreased the expression of steroidogenic acute regulatory proteins, CYP11A1 and HSD3B2 in prostate cancer cell lines, supporting the decrease of testosterone production. Human prostate cancer cell lines, LNCaP and 22Rv1 cells were incubated with or without curcumin after which cell proliferation was measured at 0, 24, 48 and 72 hours, respectively. Ciftci, O., Ozdemir, I., Tanyildizi, S., Yildiz, S., and Oguzturk, H. Antioxidative effects of curcumin, beta-myrcene and 1,8-cineole against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rats liver. El Agamy, D. S. Comparative effects of curcumin and resveratrol on aflatoxin B(1)-induced liver injury in rats.
